Journal: Materials Today Bio

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake

doi: 10.1016/j.mtbio.2026.103023

Figure Lengend Snippet: MSC-mt internalization promotes mitophagy activation under oxidative stress (A-B) Flow cytometric analysis of mitophagy in L929 cells co-cultured with fluorescently labeled MSC-mt under H 2 O 2 -induced oxidative stress. Mitophagy levels are shown for total cells as well as stratified mt transfer + and mt transfer − subpopulations, showing preferential mitophagy activation in mt transfer + cells. (C-D) Western blot analysis of mitophagy- and survival-related signaling proteins in flow-sorted mt transfer + and mt transfer − L929 cells following co-culture with fluorescently labeled MSC-mt under oxidative stress. Blots show phosphorylated PINK1 (S228), total PINK1, Parkin, total p62, phosphorylated p62 (S349 and S403), pAKT, OXPHOS components, and TOM20, highlighting enhanced PINK1–Parkin signaling and mitophagy-associated p62 processing in mt transfer + cells. (E) Flow cytometric assessment of mitophagy in total, mt transfer + , and mt transfer − populations following co-culture with PINK1-deficient MSC-derived mitochondria (siPINK1-mt) under oxidative stress, showing attenuated mitophagy activation compared with control MSC-mt. (F) Representative immunofluorescence images of L929 cells under control, H 2 O 2 , and H 2 O 2 + MSC-mt conditions, showing depolarized mitochondria (mitoPeDPP, green) and mitophagy signals (mitophagy, red), indicating increased mitophagic engagement under oxidative stress with MSC-mt transfer. Scale bar = 20 μm. (G–J) Flow cytometric analysis of depolarized mitochondria (mitoPeDPP) and mitophagy in L929 cells under H 2 O 2 stimulation with or without fluorescently labeled MSC-mt co-culture. (G) Representative flow cytometry plots. (H) Quantification of the proportions of mitoPeDPP + , mitophagy + , and double-positive cell populations. (I) Mean fluorescence intensity (MFI) of mitophagy signals, with stratification by mt transfer + and mt transfer − populations. (J) MFI of mitoPeDPP signals, with stratification by mt transfer + and mt transfer − populations. All experiments were independently repeated three times (n = 3) and representative images are shown. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Following co-culture, mitophagy was detected using a mitophagy detection kit (Dojindo, Cat# MD01), while depolarized mitochondria were simultaneously labeled with MitoPeDPP (Dojindo, Cat# M466), according to the manufacturers’ instructions.

Techniques: Activation Assay, Cell Culture, Labeling, Western Blot, Co-Culture Assay, Derivative Assay, Control, Immunofluorescence, Flow Cytometry, Fluorescence

Journal: International Journal of Pharmaceutics: X

Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition

doi: 10.1016/j.ijpx.2026.100489

Figure Lengend Snippet: In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Article Snippet: After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis.

Techniques: In Vitro, Drug discovery, Incubation, MTT Assay, Flow Cytometry, Staining, Generated

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: Exosomes derived from low-passage DPCs regulated HFSC proliferation. (A) Indirect immunofluorescence showing ALPL and PCNA expression in low-passage (P1) and high-passage (P8) DPCs (scale bar = 50 μm). (B) RT-qPCR analysis of HF development-related gene expression in P1 and P8 DPCs (unpaired two-tailed t -test, n = 3). (C) TEM images of exosomes from P1 DPCs (DPC-Exos P1) and P8 DPCs (DPC-Exos P8). (D) NTA measurement of particle size of DPC-Exos P1 and DPC-Exos P8. (E) Western blot detection of exosome-specific proteins in DPC-Exos P1 and DPC-Exos P8. (F) CCK-8 assay evaluating HFSC proliferation after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 5). (G) Flow cytometry analysis of HFSC apoptosis after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 3). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: The Annexin V-FITC Apoptosis Detection Kit (Vazyme, China, Cat No. A214) was employed, and apoptosis rates were analyzed via flow cytometry using a FACSAria SORP instrument (Becton Dickinson, USA).

Techniques: Derivative Assay, Immunofluorescence, Expressing, Quantitative RT-PCR, Gene Expression, Two Tailed Test, Western Blot, CCK-8 Assay, Flow Cytometry